How to Clean Cell Culture Ware? - MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.

How to Clean Cell Culture Ware?

Introduction to cell culture wares


A culture dish is a laboratory vessel used for cell culture experiments. It consists of a flat circular dish and a cover, usually made of glass or plastic. It was originally designed by German biologist Robert Koch based on the work of bacteriologist Julius Richard Petri in 1887, so it is also called a "Petri dish".


Cell culture vessels (cell culture consumables) are laboratory vessels used for the cultivation of microorganisms or cells. They consist of a flat, circular bottom and a lid, usually made of glass or plastic. The materials of the culture dishes are mainly divided into two categories: glass and plastic. Glass dishes can be used for plant materials, microbial cultures, and adherence culture of animal cells. Plastic dishes may be made of polyethylene material and are available in disposable or reusable forms. They are suitable for laboratory inoculation, line drawing, and bacterial separation operations, and can be used for plant material culture.


Cleaning methods for cell culture wares


Cell culture vessels are commonly used vessels for the culture of microorganisms and cells, and are common medical laboratory consumables. The materials of cell culture wares are mainly glass and plastic, which are suitable for inoculation, line drawing, separation and other operations. However, cell culture wares may produce some dirt during use, which can affect the cultivation of cells and microorganisms. The cleaning method for cell culture wares generally goes through four steps: soaking, brushing, acid soaking, and cleaning.


Soaking: 


New or used glassware should be soaked in clean water first to soften and dissolve the adhered substances. New glassware must be washed briefly with tap water before use, and then soaked in 5% hydrochloric acid overnight; Used glassware often has a lot of protein and oil attached to it, which is not easy to remove by brushing when dried, so it should be soaked in clean water immediately after use for subsequent brushing.


Brushing: 


Put the soaked glassware into a detergent solution and repeatedly brush it with a soft-bristled brush. Don't leave any blind spots and prevent the surface finish of the vessel from being damaged. Rinse the cleaned glassware thoroughly with water, and dry it for soaking in acid.


Acid soaking: 


Acid soaking is to immerse the above vessel in a cleaning solution, also called acid solution, to remove any possible remaining substances on the vessel's surface through the strong oxidation effect of the acid solution. The acid soaking should not be less than six hours, generally overnight or longer, and the vessel should be handled with care when taken out.


Rinsing: 


Both the brushed and acid soaked cell culture wares must be washed thoroughly with water. Whether the vessel is rinsed clean after acid soaking directly affects the success of cell cultures. For manually cleaned acid-soaked vessels, each vessel should be rinsed at least 15 times with the "water injection-emptying" method, and finally soaked and washed with distilled water for 2 to 3 times, dried or dried, and packaged for later use.