Cell culture is a method of simulating the in vivo environment in vitro to enable cells to survive, grow, multiply, and maintain their primary structure and function. Cell culture, also known as cell cloning technology, is a formal term for cell culture technology in biology. Whether for the entire biotechnology field or one of its areas of biological cloning technology, cell culture is an essential process and an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and research on signal transduction, cellular synthesis metabolism, cellular growth proliferation and other aspects can be conducted. In vitro culture is highly dependent on culture systems and environment. This article analyzes the conventional cell culture system - culture vessels.
Cell culture consumables are commonly used glass instruments mainly used for microbial or cell culture. They consist of a flat, circular bottom and a cover, generally made of glass or plastic. Glass cell culture vessels can be used for plant materials, microbial culture, and animal cell adherent culture. Plastic cell culture vessels are generally made of polyethylene materials and are available for disposable and multiple uses. They are suitable for laboratory inoculation, line drawing, and bacteria separation, and can be used for plant material culture.
Cell culture ware is fragile and easily broken. Therefore, they should be handled carefully when cleaning and handling, and are put down gently. After use, the culture vessels should be cleaned and stored in a safe and fixed place to prevent damage or breakage.
Cell culture wares are mainly used for glass or plastic circular vessels that carry liquid culture media or solid agar culture media for cell culture. They are mainly suitable for epidemic prevention stations, hospitals, biological products, food industry, pharmaceutical industry, etc. for bacterial isolation, culture, antibiotic potency testing and qualitative analysis. In agricultural, aquatic and other scientific research, they are used for the artificial culture and hatching research of seeds, plants, insects, and fish species. In the electronics industry or other industries, they are used as containers.
Of course, a more rigorous operation would be to use one hand, usually the left hand, to open the lid of the culture vessel at an angle with a sterile long pipette to remove the culture medium. Then, replace the medium with fresh culture medium, preferably not blowing directly onto the cells, but blowing towards the side wall of the culture vessel to make it flow into the cell culture vessel. It is better to be close to the alcohol lamp, but not too close, as plastic is prone to deformation when exposed to heat.