What Are the Important Features of Pipette Tips? - MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.

What Are the Important Features of Pipette Tips?

1. Hydrophobicity of pipette tips

Hydrophobic pipette tips prevent aerosol contamination from coming into contact with the pipette stem. Therefore, these tips are best suited for applications that require a high degree of sterility, including radioisotope processing and DNA amplification. In such tips, unique polypropylene blends are used as molds with pronounced hydrophobic properties. This allows liquids with low surface tension to diffuse and wet the inner surface of the tip, allowing all sample to be removed from the tip.

This pipette tip feature maximizes the range of sample recovery from the pipette, improving the precision of pipetting solutions containing detergents or any other fluid with low surface tension. Additionally, this tip provides superior chemical resistance without the risk of leachables. They can be used in highly sensitive biological studies, including the use of viscous reagents and/or detergents. Such applications include sequencing, PCR and RT-PCR, protein analysis/purification experiments, SDS-PAGE, and more.

2. Purity and scale of pipette tips

Purity: High quality pipette tips made by a professional pipette tips supplier are free of detectable DNase, RNase, PCB inhibitors, pyrogens and other types of bioburden. Pipette tips need to be sterilized to eliminate the risk of biological contamination. Usually, this is achieved through the use of radiation. However, radiation alone does not ensure that pipette tips are completely sterile and free of endotoxins or nucleic acids. For various bioassays, the purity and sterility of the equipment are absolutely critical. Needless to say, manufacturers who supply high-quality pipette tips follow cleaning protocols during production and ensure that the bulk packaging is free of any type of contamination.

Scale: There are scale marks on the side of the tip for pipette tips to measure different liquid volumes. These tips act as reliable secondary scales and ensure that the volume of liquid is absolutely correct.

3. Autoclave sterilization of pipette tips

Autoclaving is a process used to sterilize laboratory equipment such as pipette tips. Sterilization is achieved by passing superheated steam through laboratory utensils. This is done by removing air with the help of a vacuum pump or down steam displacement. Some types of pipette tips on the market are compatible with autoclaves, while others are not. These tips are manufactured from autoclavable materials. However, most companies have strict procedures to follow during autoclaving (121°C, 20 minutes). However, sometimes autoclaving is not effective in eliminating certain heat-resistant microorganisms or enzymatic proteins, such as RNases, which are difficult to inactivate even when exposed to high temperatures.

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