What Should Be Avoided When Using a Cryovial Tube? - MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.
MDHC Life Technologies (Kunshan) Co., Ltd.

What Should Be Avoided When Using a Cryovial Tube?

Differences of cryovial tubes with other types of small bottles


100% Sterile: 


In order to ensure that the freezing tubes are free from contamination, they are sterilized through a process that meets the SAL 10-6 standard specified by DIN EN ISO11137. All small bottles have a sterile period of more than five years. In addition to the sterilization property, they are also stored in contamination-free freezing packaging and do not contain detectable heavy metals. Moreover, each freezing tube undergoes quality checks for BSE/TSE, detectable RNase, DNase, and cytotoxins. Using low-temperature bottles means that your samples can be protected from contamination caused by packaging.


Leak-Proof: 


A unique feature of freezing tubes is the external thread design, which reduces the chance of leakage without increasing the volume of the freezing tube. Just half-turn can seal the leap, achieving completely leak-proof quality.


Durability: 


It is made of unique propylene material with a pressure range of approximately 95kPa/0.95bar. The material design is long-lasting and can withstand severe temperature, pressure, and energy conditions.


Things to avoid when using freezing tubes


Cryovial tubes made by professional laboratory consumables manufacturers are mainly used to store biological materials at low temperatures using gaseous liquid nitrogen. When removing small bottles with samples from low temperatures, liquid nitrogen may seep in, causing the small bottle to expand as it transitions from liquid to gas. Mishandling can cause the freezing tube to explode due to rapid expansion and contraction caused by temperature differences.


Once nitrogen seeps in, it produces an interior temperature colder than the external room temperature. The sudden temperature change causes the tube to rapidly expand and contract, leading to tube explosion. Exploded small bottles can cause unprecedented injury and exposure to contents in the small bottle. In addition, there may be cross-contamination of contents or cells, which can damage your experiment. After introducing the sample into the freezing tube, make sure to tighten the lid. Store the small bottle in a tamper-proof rack to avoid damage.


Here are some corrective measures you can take to avoid freezing tube explosions: Switch from freezing tubes with internal threads and mother caps to freezing tubes with external caps. Ensure that you follow the freezing tube manufacturer's instructions, stating that external and internal thread small bottles should not be immersed in liquid nitrogen to avoid the risk of explosion. Avoid storing small bottles containing biohazardous material in storage units that use liquid nitrogen, rather than storing them in units that use gaseous liquid nitrogen. When using freezing tubes exposed to liquid nitrogen, immediately place the freezing tube in a resilient and well-sealed plastic bag before thawing. This will help control explosions if any liquid nitrogen seeps in. You can also choose to transfer small bottles from liquid to gas, which takes about 24 hours to thaw, and there is no risk of explosion.

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