MDHC cell culture series often a solution for every step of your workflow. MDHC cell culture series include cell culture flasks, cell culture dishes, cell culture plates and cell culture slides. The products are all made of high clarity, 100% virgin polystyrene and sterilized by E-beam, which provides high-quality lab experiment experience.
For cell culturing, here are some precautions for the labs:
Before you can grow cells, you need to do these things:
1. Treatment of consumables
For the cleaning of glassware, glassware (cell bottles, nutrient bottles, small penicillin bottles, etc.) should be cleaned with laundry (pay attention to dead spots), and then rinsed. They were soaked in acid solution for more than 24 h, and then washed several times with clean water to remove the residual acid solution. Bottles and things like that. Shake them vigorously during the rinsing process.
2. Configuration of reagents
For reagent configuration, the liquid used for cell culture is generally double steamed water. Attention should also be paid to the regulation of pH.
3. Sterility test
Asepsis test mainly uses filtration to remove bacteria: such as trypsin, antibiotics, G-418 solution, various media, sodium pyruvate, glutamine, etc. Some can be sterilized by autoclaving: PBS, D-Hank's, etc.
Different cells have different requirements for medium and should be used according to their own cell requirements.
Asepsis is the most important step in cell culture
Before the experiment, the ultra-clean table was irradiated with UV lamp for 30 minutes to sterilize, the sterile operating table was wiped with 70% ethanol, and the ultra-clean table fan was turned on to run for several minutes before the experimental operation began.
Only one cell line was treated per operation to avoid cross-contamination between cells.
At the end of the experiment, the experimental items were taken out of the worktable and the sterile operating table was wiped again with 70 % ethanol. The experimental supplies were wiped with 70% ethanol before being brought into the sterile operating table. The experimental operation should be performed in the central sterile area and generally not in the marginal area.
Late stage of cell culture——Periodic observation
You'd better visit them every day to see how they're growing：
1. Check the change of color and transparency of the culture medium. The color turns yellow, indicating that the PH value decreases; Red or purplish red, indicating that the PH value rises, cell growth arrest, death, generally stable growth cells can be changed once every 2-3 days, slow growth cells can be changed once every 3-4 days.
2. Observe the growth status of cells. The cells cover 80% of the bottom of the bottle and should be passaged in time.
3．Observe the changes of cell morphology, cell transparency, strong refraction, clear outline is better.
4．Pay attention to microbial contamination, often appear in the culture medium turbidity, floating hyphae or cell bacteria in the liquid, not only refers to microorganisms, including all mixed into the culture environment of the cell survival of harmful components and cause cell impurity of foreign bodies and cells.